Anti-PSME2 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-PSME2 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-PSME2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-PSME2 Magnetic Beads-IP Kit Product Description
The Anti-PSME2 magnetic Beads, conjugated with Anti-PSME2 antibody, are used for immuneprecipitation (IP) of PSME2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PSME2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PSME2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-PSME2 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human PSME2 / PA28b protein (Catalog#14640-H07E)
Species Reactivity
Human PSME2 / PA28b
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human PSME2 / PA28b (rh PSME2 / PA28b; Catalog#14640-H07E; PSME2_HUMAN; Met1-Tyr239). PSME2 / PA28b specific IgG was purified by Human PSME2 / PA28b affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-PSME2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-PA28BALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PA28betaALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-REGbetaALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
PSME2 Background Information
PSME2, also known as PA28b, is a subunit of proteasome. The 26S proteasome multicatalytic proteinase complex has a highly ordered structure composed of 2 complexes, a 2S core and a 19S regulator. The 2S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 1 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME2 gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring.
Full Name
proteasome (prosome, macropain) activator subunit 2 (PA28 beta)
References
Ahn K. et al., 1996, J Biol Chem. 271 (30): 18237-42. Rual Jean-François. et al., 2005, Nature. 437 (7062): 1173-8. Ahn JY. et al., 1995, FEBS Lett. 366 (1): 37-42.
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