Anti-C1orf33 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-C1orf33 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-C1orf33 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-C1orf33 Magnetic Beads-IP Kit Product Description
The Anti-C1orf33 magnetic Beads, conjugated with Anti-C1orf33 antibody, are used for immuneprecipitation (IP) of C1orf33 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing C1orf33 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound C1orf33 proteins are dissociated from the magnetic beads using an elution buffer. Anti-C1orf33 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human MRTO4 / MRT4 protein (Catalog#14639-H07E)
Species Reactivity
Human MRTO4 / MRT4
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human MRTO4 / MRT4 (rh MRTO4 / MRT4; Catalog#14639-H07E; Q9UKD2; Met1-Asp239). MRTO4 / MRT4 specific IgG was purified by Human MRTO4 / MRT4 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-C1orf33 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-C1orf33ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-dJ657E11.4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MRT4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
C1orf33 Background Information
MRTO4, also known as MRT4, belongs to the ribosomal protein L1P family. MRTO4 is a ribosomal P-like protein showing extensive sequence similarity to the ribosomal P protein. The precise function of MRTO4 is currently unknown. It appears to be involved in mRNA turnover and ribosome assembly. MRTO4 acts as a trans-acting factor which modulates the assembly of the pre-6S particle.
Full Name
MRT4 homolog, ribosome maturation factor
References
Zhao J. et al., 2011, J Proteomics. 75 (2): 588-602. Havugimana PC. et al., 2012, Cell. 150 (5): 1068-81. Wu Z. et al., 2012, PLoS One. 7 (8): e43997. Michalec B. et al., 2010, Int J Biochem Cell Biol. 42 (5): 736-48.
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