Anti-ATP5D Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-ATP5D Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-ATP5D magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-ATP5D Magnetic Beads-IP Kit Product Description
The Anti-ATP5D magnetic Beads, conjugated with Anti-ATP5D antibody, are used for immuneprecipitation (IP) of ATP5D proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ATP5D proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ATP5D proteins are dissociated from the magnetic beads using an elution buffer. Anti-ATP5D Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human ATP5D protein (Catalog#14630-H07E)
Species Reactivity
Human ATP5D
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human ATP5D (rh ATP5D; Catalog#14630-H07E; P30049; Ala23-Glu168). ATP5D specific IgG was purified by Human ATP5D affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
ATP5D Background Information
ATP5D is a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase consists of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5D gene encodes the delta subunit of the catalytic core.
Full Name
ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit
References
Jordan EM. et al., 1992, Biochim Biophys Acta 1130 (1): 123-6. Yoshida M. et al., 2001, Nat Rev Mol Cell Biol. 2 (9): 669-77. Hochstrasser DF. et al., 1993, Electrophoresis. 13 (12): 992-1001.