Anti-EEF1B2 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-EEF1B2 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-EEF1B2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-EEF1B2 Magnetic Beads-IP Kit Product Description
The Anti-EEF1B2 magnetic Beads, conjugated with Anti-EEF1B2 antibody, are used for immuneprecipitation (IP) of EEF1B2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing EEF1B2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound EEF1B2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-EEF1B2 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human EF1B / EEF1B2 protein (Catalog#14611-H07E)
Species Reactivity
Human EF1B / EEF1B2
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human EF1B / EEF1B2 (rh EF1B / EEF1B2; Catalog#14611-H07E; P24534; Met1-Ile225). EF1B / EEF1B2 specific IgG was purified by Human EF1B / EEF1B2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-EEF1B2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-EEF1BALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-EEF1B1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-EF1BALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
EEF1B2 Background Information
EF1B, also known as EEF1B2, is a translation elongation factor. It belongs to the EF-1-beta/EF-1-delta family. Elongation factors are a set of proteins that are used in protein synthesis in the cell. In the ribosome, they facilitate translational elongation, from the formation of the first peptide bond to the formation of the last one. EF1B is more complex in eukaryotes than in bacteria, and consists of three subunits: EF1B-alpha, EF1B-gamma and EF1B-beta. EF1B contains 1 GST C-terminal domain. It is involved in the transfer of aminoacylated tRNAs to the ribosome. EF1B is required to regenerate EF1A from its inactive form (EF1A-GDP) to its active form (EF1A-GTP). EF1A is then ready to interact with a new aminoacyl-tRNA to begin the cycle again.
Full Name
eukaryotic translation elongation factor 1 beta 2
References
Pizzuti A. et al., 1994, Biochem Biophys Res Commun. 197 (1): 154-62. Rual. et al., 2005, Nature. 437 (7062): 1173-8. Stelzl. et al., 2005, Cell. 122 (6): 957-68. Sang Lee. et al., 2002, Biochem Biophys Res Commun. 291 (1): 158-64.
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