Anti-XTP3TPA Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-XTP3TPA Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-XTP3TPA magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-XTP3TPA Magnetic Beads-IP Kit Product Description
The Anti-XTP3TPA magnetic Beads, conjugated with Anti-XTP3TPA antibody, are used for immuneprecipitation (IP) of XTP3TPA proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing XTP3TPA proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound XTP3TPA proteins are dissociated from the magnetic beads using an elution buffer. Anti-XTP3TPA Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human XTP3TPA / DCTPP1 protein (Catalog#14577-H07E)
Species Reactivity
Human XTP3TPA / DCTPP1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human XTP3TPA / DCTPP1 (rh XTP3TPA / DCTPP1; Catalog#14577-H07E; NP_077001.1; Met1-Thr170). XTP3TPA / DCTPP1 specific IgG was purified by Human XTP3TPA / DCTPP1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-XTP3TPA Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CDA03ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-RS21C6ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-XTP3TPAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
XTP3TPA Background Information
DCTPP1 hydrolyzes deoxynucleoside triphosphates (dNTPs) to the corresponding nucleoside monophosphates. It has a strong preference for modified dCTP. DCTPP1’s activity is highest with 5-iodo-dCTP, followed by 5-bromo-dCTP, unmodified dCTP, 5-methyl-dCTP and 5-chloro-dCTP. DCTPP1 also hydrolyzes 2-chloro-dATP and 2-hydroxy-dATP with lower efficiency, and has even lower activity with unmodified dATP, dTTP and dUTP (in vitro). DCTPP1 does not hydrolyze ATP, UTP, ITP, GTP, dADP, dCDP or dGTP. It may protect DNA or RNA against the incorporation of non-canonical nucleotide triphosphates. DCTPP1 may also protect cells against inappropriate methylation of CpG islands by DNA methyltransferases.
Full Name
dCTP pyrophosphatase 1
References
Stelzl U. et al., 2005, Cell. 122 (6): 957-68. Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903. Moroz OV. et al., 2005, J Mol Biol. 347 (2): 243-55.
新浪微博
腾讯微博
QQ空间
客服
