Anti-CBFB Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-CBFB Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CBFB magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CBFB Magnetic Beads-IP Kit Product Description
The Anti-CBFB magnetic Beads, conjugated with Anti-CBFB antibody, are used for immuneprecipitation (IP) of CBFB proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CBFB proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CBFB proteins are dissociated from the magnetic beads using an elution buffer. Anti-CBFB Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CBFB / CBF-beta protein (Catalog#14569-H07E)
Species Reactivity
Human CBFB / CBF-beta
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CBFB / CBF-beta (rh CBFB / CBF-beta; Catalog#14569-H07E; Q13951-1; Met1-Pro182). CBFB / CBF-beta specific IgG was purified by Human CBFB / CBF-beta affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CBFB Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-PEBP2BALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CBFB Background Information
CBFB is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). CBFB is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for CBFB gene. Mutations in CBFB are implicated in cases of breast cancer.
Full Name
core-binding factor, beta subunit
References
Hart SM. et al., 2003, Haematologica. 87(12): 1307-23. Liu P. et al., 1995, Cold Spring Harb Symp Quant Biol. 59: 547-53. Liu P. et al., 1993, Science. 261 (5124): 1041-4.