Anti-ECH1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-ECH1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-ECH1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-ECH1 Magnetic Beads-IP Kit Product Description
The Anti-ECH1 magnetic Beads, conjugated with Anti-ECH1 antibody, are used for immuneprecipitation (IP) of ECH1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ECH1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ECH1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-ECH1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human ECH1 protein (Catalog#14566-H07E)
Species Reactivity
Human ECH1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human ECH1 (rh ECH1; Catalog#14566-H07E; Q13011; Thr34-Leu328). ECH1 specific IgG was purified by Human ECH1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-ECH1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-HPXELALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
ECH1 Background Information
ECH1 is a member of the hydratase/isomerase superfamily. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. ECH1 contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. ECH1 functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.
Full Name
enoyl CoA hydratase 1, peroxisomal
References
Kovalyov LI, et al. (2006) Polymorphism of delta3,5-delta2,4-dienoyl-coenzyme A isomerase (the ECH1 gene product protein) in human striated muscle tissue. Biochemistry Mosc. 71(4): 448-53. Olsen JV, et al. (2006) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 127(3):635-48. FitzPatrick DR, et al. (1995) Isolation and characterization of rat and human cDNAs encoding a novel putative peroxisomal enoyl-CoA hydratase. Genomics. 27(3):457-66.
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