Anti-DCXR Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-DCXR Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-DCXR magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-DCXR Magnetic Beads-IP Kit Product Description
The Anti-DCXR magnetic Beads, conjugated with Anti-DCXR antibody, are used for immuneprecipitation (IP) of DCXR proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing DCXR proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound DCXR proteins are dissociated from the magnetic beads using an elution buffer. Anti-DCXR Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human DCXR / HCR2 protein (Catalog#14562-H07E)
Species Reactivity
Human DCXR / HCR2
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human DCXR / HCR2 (rh DCXR / HCR2; Catalog#14562-H07E; Q7Z4W1; Met1-Cys244). DCXR / HCR2 specific IgG was purified by Human DCXR / HCR2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-DCXR Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-DCRALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HCR2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HCRIIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-KIDCRALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-P34HALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PNTSUALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SDR20C1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-XRALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
DCXR Background Information
DCXR, also known as HCR2, belongs to the short-chain dehydrogenases/reductases (SDR) family. It is highly expressed in kidney, liver and epididymis. In the epididymis, DCXR is mainly expressed in the proximal and distal sections of the corpus region. HCR2 is weakly or not expressed in brain, lung, heart, spleen and testis. DCXR catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L-xylulose. DCXR participates in the uronate cycle of glucose metabolism. It may play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules.
Full Name
dicarbonyl/L-xylulose reductase
References
Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 44(2):325-40. Pierce SB, et al. (2011) Garrod's fourth inborn error of metabolism solved by the identification of mutations causing pentosuria. Proc Natl Acad Sci. 108(45):18313-7. Udeshi ND, et al. (2012) Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition. Mol Cell Proteomics. 11(5):148-59.
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