Anti-LC3B Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-LC3B Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-LC3B magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-LC3B Magnetic Beads-IP Kit Product Description
The Anti-LC3B magnetic Beads, conjugated with Anti-LC3B antibody, are used for immuneprecipitation (IP) of LC3B proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing LC3B proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound LC3B proteins are dissociated from the magnetic beads using an elution buffer. Anti-LC3B Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human LC3B / MAP1LC3B protein (Catalog#14555-H07E)
Species Reactivity
Human LC3B / MAP1LC3B
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human LC3B / MAP1LC3B (rh LC3B / MAP1LC3B; Catalog#14555-H07E; Q9GZQ8; Met1-Val125). LC3B / MAP1LC3B specific IgG was purified by Human LC3B / MAP1LC3B affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-LC3B Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ATG8FALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-LC3BALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MAP1A/1BLC3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MAP1LC3B-aALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
LC3B Background Information
LC3B, also known as MAP1LC3B, is a member of the MAP1 LC3 family. It is moat abundantly expressed in heart, brain, skeletal muscle and testis. LC3B is a subunit of neuronal microtubule and functions in formation of autophagosomal vacuoles (autophagosomes). It associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. LC3B also plays a role in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Full Name
microtubule-associated protein 1 light chain 3 beta
References
Behrends C. et al., 2010, Nature. 466 (7302): 68-76. Tanida I. et al., 2005, Int J Biochem Cell Biol. 36 (12): 2503-18. Kabeya Y. et al., 2000, EMBO J. 19 (21): 5720-8. Cherra SJ. et al., 2010, J Cell Biol. 190 (4): 533-9.