Anti-SR1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-SR1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-SR1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-SR1 Magnetic Beads-IP Kit Product Description
The Anti-SR1 magnetic Beads, conjugated with Anti-SR1 antibody, are used for immuneprecipitation (IP) of SR1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing SR1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound SR1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-SR1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human Sorcin / SRI Protein (Catalog#14547-H20B)
Species Reactivity
Human Sorcin / SRI
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human Sorcin / SRI (rh Sorcin / SRI; Catalog#14547-H20B; P30626; Met1-Val198). Sorcin / SRI specific IgG was purified by Human Sorcin / SRI affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-SR1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CP-22ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CP22ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SCNALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-V19ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
SR1 Background Information
Sorcin was originally identified in multidrug-resistant cells. It is a alcium-binding protein. Sorcin modulates excitation-contraction coupling in the heart, contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Sorcin is overexpressed in the multi-drug resistant chinese hamster ovary cell line CHRC5 and a variety of multidrug-resistant tumor cell lines, but overexpression is not a sufficient or necessary condition for the acquisition of the multidrug-resistant phenotype.
References
Meyers MB, et al. (1995) Association of sorcin with the cardiac ryanodine receptor. J Biol Chem. 270(44):26411-8. Brownawell AM, et al. (1997) Calcium-dependent binding of sorcin to the N-terminal domain of synexin (annexin VII). J Biol Chem. 272(35):22182-90. Hansen, et al. (2003) The PEF family proteins sorcin and grancalcin interact in vivo and in vitro. FEBS Lett. 545(2-3):151-4.
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