Anti-Cofilin Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Cofilin Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Cofilin magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Cofilin Magnetic Beads-IP Kit Product Description
The Anti-Cofilin magnetic Beads, conjugated with Anti-Cofilin antibody, are used for immuneprecipitation (IP) of Cofilin proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Cofilin proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Cofilin proteins are dissociated from the magnetic beads using an elution buffer. Anti-Cofilin Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CFL1 / cofilin Protein (Catalog#14544-H07E)
Species Reactivity
Human CFL1 / cofilin
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CFL1 / cofilin (rh CFL1 / cofilin; Catalog#14544-H07E; P23528; Met1-Leu166). CFL1 / cofilin specific IgG was purified by Human CFL1 / cofilin affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Cofilin Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CFLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HEL-S-15ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Cofilin Background Information
CFL1, also known as n-cofilin, is a member of the ADF/Cofilin family. This family comprises three genes: CFL1, CFL2 and DSTN (destrin). ADF/Cofilin family members bind G-actin monomers and depolymerize actin filaments through two mechanisms: severing and increasing the off-rate for actin monomers from the pointed end. Cofilin also binds with other proteins such as myosin, tropomyosin, α-actinin, gelsolin and scruin. These proteins compete with cofilin for actin binding. Сofilin also plays a role in innate immune response. CFL1 contains 1 ADF-H domain and is widely distributed in various tissues. It is important for normal progress through mitosis and normal cytokinesis.
Full Name
cofilin 1 (non-muscle)
References
Lappalainen P. et al., 1997, Nature. 388 (6637): 78-82. Ichetovkin I. et al., 2000, Cell Motil. 45 (4): 293-306. Carlier MF. et al., 1997, J Cell Biol. 136 (6): 1307-22.
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