Anti-Optineurin Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Optineurin Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Optineurin magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Optineurin Magnetic Beads-IP Kit Product Description
The Anti-Optineurin magnetic Beads, conjugated with Anti-Optineurin antibody, are used for immuneprecipitation (IP) of Optineurin proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Optineurin proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Optineurin proteins are dissociated from the magnetic beads using an elution buffer. Anti-Optineurin Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human Optineurin / OPTN Protein (Catalog#14478-H20B)
Species Reactivity
Human Optineurin / OPTN
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human Optineurin / OPTN (rh Optineurin / OPTN; Catalog#14478-H20B; NP_068815.2; Met1-Ile577). Optineurin / OPTN specific IgG was purified by Human Optineurin / OPTN affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Optineurin Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ALS12ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-FIP2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GLC1EALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HIP7ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HYPLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NRPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TFIIIA-INTPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Optineurin Background Information
Optineurin gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein.
References
Rezaie T. et al., 2002, Science. 295 (5557): 1077-9. Li Y. et al., 1998, Mol Cell Biol. 18 (3): 1601-10. Skarnes Wc RB. et al., 2011, Nature. 474 (7351): 337-42.
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