Anti-SRFBP1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-SRFBP1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-SRFBP1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-SRFBP1 Magnetic Beads-IP Kit Product Description
The Anti-SRFBP1 magnetic Beads, conjugated with Anti-SRFBP1 antibody, are used for immuneprecipitation (IP) of SRFBP1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing SRFBP1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound SRFBP1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-SRFBP1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human SRFBP1 (p49 / STRAP) protein (Catalog#14461-H20B)
Species Reactivity
Human SRFBP1 (p49 / STRAP)
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human SRFBP1 (p49 / STRAP) (rh SRFBP1 (p49 / STRAP); Catalog#14461-H20B; Q8NEF9; Met1-Asp429). SRFBP1 (p49 / STRAP) specific IgG was purified by Human SRFBP1 (p49 / STRAP) affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-SRFBP1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-BUD22ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-P49ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-p49/STRAPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Rlb1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-STRAPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
SRFBP1 Background Information
SRFBP1 contains 7 WD repeats and belongs to the WD repeat STRAP family. SRFBP1 may play a role in the cellular distribution of the SMN complex. The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. SRFBP1 negatively regulates TGF-beta signaling but positively regulates the PDPK1 kinase activity by enhancing its autophosphorylation and by significantly reducing the association of PDPK1 with 14-3-3 protein. SRFBP1 may be involved in regulating transcriptional activation of cardiac genes during the aging process. It also may play a role in biosynthesis and/or processing of SLC2A4 in adipose cells.
Full Name
serum response factor binding protein 1
References
Datta PK, et al. (1999) Identification of STRAP, a novel WD domain protein in transforming growth factor-beta signaling. J Biol Chem. 273(52):34671-4. Datta PK, et al. (1998) Identification of STRAP, a novel WD domain protein in transforming growth factor-beta signaling. J Biol Chem. 273(52):34671-4. Datta PK, et al. (2000) STRAP and Smad7 synergize in the inhibition of transforming growth factor beta signaling. Mol Cell Biol. 20(9):3157-67.
新浪微博
腾讯微博
QQ空间
客服
