Anti-Creatine kinase B Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Creatine kinase B Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Creatine kinase B magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Creatine kinase B Magnetic Beads-IP Kit Product Description
The Anti-Creatine kinase B magnetic Beads, conjugated with Anti-Creatine kinase B antibody, are used for immuneprecipitation (IP) of Creatine kinase B proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Creatine kinase B proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Creatine kinase B proteins are dissociated from the magnetic beads using an elution buffer. Anti-Creatine kinase B Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CKB / Creatine kinase B type protein (Catalog#14415-H07B)
Species Reactivity
Human CKB / Creatine kinase B
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CKB / Creatine kinase B type (rh CKB / Creatine kinase B type; Catalog#14415-H07B; P12277; Pro2-Lys381). CKB / Creatine kinase B type specific IgG was purified by Human CKB / Creatine kinase B type affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Creatine kinase B Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-B-CKALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-BCKALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CKBBALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HEL-211ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HEL-S-29ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Creatine kinase B Background Information
CKB(Creatine kinase B type) contains 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. It belongs to the ATP:guanido phosphotransferase family. CKB consists of a homodimer of two identical brain-type CK-B subunits. CKB is a cytoplasmic enzyme involved in cellular energy homeostasis, with certain fractions of the enzyme being bound to cell membranes, ATPases, and a variety of ATP-requiring enzymes in the cell. There, CKB forms tightly coupled microcompartments for in situ regeneration of ATP that has been used up. CKB reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine or between phospho-creatine (PCr) and ADP. Its functional entity is a homodimer in brain, smooth muscle as well as in other tissues and cells such as neuronal cells, retina, kidney, bone etc.
Full Name
creatine kinase, brain
References
Wienker TF, et al. (1985) A dominant mutation causing ectopic expression of the creatine kinase B gene maps on chromosome 14. Cytogenet Cell Genet. 40:776. Mariman EC, et al. (1989) Complete nucleotide sequence of the human creatine kinase B gene. Nucleic Acids Res. 17(15):6385. Bong S, et al. (2008) Structural studies of human brain-type creatine kinase complexed with the ADP–Mg2+–NO3?–creatine transition-state analogue complex. FEBS Letters. 582(28): 3959-65.
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