Anti-GGCT Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-GGCT Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-GGCT magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-GGCT Magnetic Beads-IP Kit Product Description
The Anti-GGCT magnetic Beads, conjugated with Anti-GGCT antibody, are used for immuneprecipitation (IP) of GGCT proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing GGCT proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound GGCT proteins are dissociated from the magnetic beads using an elution buffer. Anti-GGCT Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human GGCT protein (Catalog#14366-H07E)
Species Reactivity
Human GGCT
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human GGCT (rh GGCT; Catalog#14366-H07E; O75223-1; Met1-Leu188). GGCT specific IgG was purified by Human GGCT affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-GGCT Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-C7orf24ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CRF21ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GCTGALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GGCALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
GGCT Background Information
GGCT belongs to the gamma-glutamylcyclotransferase family. It catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides, the penultimate step in glutathione catabolism. GGCT may play a significant role in glutathione homeostasis. GGCT also induces release of cytochrome c from mitochondria with resultant induction of apoptosis. Pseudogenes of GGCT gene are located on the long arm of chromosome 5 and the short arm of chromosomes 2 and 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Full Name
gamma-glutamylcyclotransferase
References
Wagner SA. et al., 2011, Mol Cell Proteomics. 10 (10): M111.013284. Kim W. et al., 2011, Mol Cell. 44 (2): 325-40. Amano T. et al., 2012, J Histochem Cytochem. 60 (1): 76-86.
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