Anti-PFDN2 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-PFDN2 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-PFDN2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-PFDN2 Magnetic Beads-IP Kit Product Description
The Anti-PFDN2 magnetic Beads, conjugated with Anti-PFDN2 antibody, are used for immuneprecipitation (IP) of PFDN2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PFDN2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PFDN2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-PFDN2 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human PFD2 / PFDN2 protein (Catalog#14349-H07E)
Species Reactivity
Human PFD2 / PFDN2
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human PFD2 / PFDN2 (rh PFD2 / PFDN2; Catalog#14349-H07E; Q9UHV9; Met1-Ser154). PFD2 / PFDN2 specific IgG was purified by Human PFD2 / PFDN2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-PFDN2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-PFD2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
PFDN2 Background Information
PFD2, also known as PFDN2, belongs to the prefoldin beta subunit family. PFD2 is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. PFD2 binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. PFD2 also binds to a nascent polypeptide chain and promotes folding in an setting in which there are many competing pathways for nonnative proteins.
Full Name
prefoldin subunit 2
References
Gerhard DS, et al. (2004) The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) . Genome Res. 14(10B):2121-7. Bauch A, et al. (2004) A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway. Nat Cell Biol. 6(2):97-105. Nogaj LA, et al. (2005) Cellular levels of glutamyl-tRNA reductase and glutamate-1-semialdehyde aminotransferase do not control chlorophyll synthesis in Chlamydomonas reinhardtii. Plant Physiol. 139(1):389-96.
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