Anti-CARHSP1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CARHSP1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CARHSP1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CARHSP1 Magnetic Beads-IP Kit Product Description
The Anti-CARHSP1 magnetic Beads, conjugated with Anti-CARHSP1 antibody, are used for immuneprecipitation (IP) of CARHSP1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CARHSP1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CARHSP1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CARHSP1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CARHSP1 protein (Catalog#14333-H07E)
Species Reactivity
Human CARHSP1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CARHSP1 (rh CARHSP1; Catalog#14333-H07E; Q9Y2V2; Met1-Ser147). CARHSP1 specific IgG was purified by Human CARHSP1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CARHSP1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CRHSP-24ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CSDC1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CARHSP1 Background Information
CARHSP1 is a biomarker for diabetic complications. Adenovirus-mediated CARHSP1 overexpression and siRNA-mediated knockdown experiments were performed to characterize the role of CARHSP1 in the regulation of gluconeogenic gene expression. CARHSP1 is regulated by nutrient status in the liver and functions at the transcriptional level to negatively regulate gluconeogenic genes, including the glucose-6-phosphatase catalytic subunit (G6Pc) and phosphoenolpyruvate carboxykinase 1 (PEPCK1). In addition, it is found that CARHSP1 can physically interact with peroxisome proliferator-activated receptor-α (PPARα) and inhibit its transcriptional activity. Both pharmacological and genetic ablations of PPARα attenuate the inhibitory effect of CARHSP1 on gluconeogenic gene expression in hepatocytes.
Full Name
calcium regulated heat stable protein 1, 24kDa
References
Wistow G. et al., 2002, Mol Vis. 8: 205-20. Wishart MJ. et al., 2002, Proc Natl Acad Sci. 99 (4): 2112-7. Groblewski GE. et al., 1998, J Biol Chem. 273 (35): 22738-44. Fan Y. et al., 2011, J Biol Chem. 286 (47): 40584-94.
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