Anti-PC4 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-PC4 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-PC4 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-PC4 Magnetic Beads-IP Kit Product Description
The Anti-PC4 magnetic Beads, conjugated with Anti-PC4 antibody, are used for immuneprecipitation (IP) of PC4 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PC4 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PC4 proteins are dissociated from the magnetic beads using an elution buffer. Anti-PC4 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human SUB1 / PC4 protein (Catalog#14326-H07E)
Species Reactivity
Human SUB1 / PC4
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human SUB1 / PC4 (rh SUB1 / PC4; Catalog#14326-H07E; P53999; Met1-Leu127). SUB1 / PC4 specific IgG was purified by Human SUB1 / PC4 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-PC4 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-p14ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-P15ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PC4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-RPO2TC1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SUB1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
PC4 Background Information
SUB1 belongs to the transcriptional coactivator PC4 family. It is a general coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. SUB1 binds single-stranded DNA. Single-stranded DNA-binding proteins play many roles in nucleic acid metabolism, but their importance during transcription remains unclear. SUB1 exhibits strong genetic interactions with factors necessary for promoter melting. It localizes near the transcription bubble in vitro and binds to promoters in vivo dependent upon preinitiation complexes assembly. SUB1 interacts with the nontemplate strand of RNApII complexes during initiation. It may also be involved in stabilizing the multiprotein transcription complex.
Full Name
SUB1 homolog, transcriptional regulator
References
Knaus R, et al. (1996) Yeast SUB1 is a suppressor of TFIIB mutations and has homology to the human co-activator PC4. EMBO J. 15(8):1933-40. Ge H, et al. (1994) Purification, cloning, and characterization of a human coactivator, PC4, that mediates transcriptional activation of class II genes. Cell. 78(3):513-23. Kaiser K, et al. (1994) A novel mediator of class II gene transcription with homology to viral immediate-early transcriptional regulators. Cell. 78(3):525-34.
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