Anti-MAP1LC3A Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-MAP1LC3A Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-MAP1LC3A magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-MAP1LC3A Magnetic Beads-IP Kit Product Description
The Anti-MAP1LC3A magnetic Beads, conjugated with Anti-MAP1LC3A antibody, are used for immuneprecipitation (IP) of MAP1LC3A proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing MAP1LC3A proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound MAP1LC3A proteins are dissociated from the magnetic beads using an elution buffer. Anti-MAP1LC3A Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human LC3A / MAP1LC3A protein (Catalog#14322-H08E)
Species Reactivity
Human LC3A / MAP1LC3A
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human LC3A / MAP1LC3A (rh LC3A / MAP1LC3A; Catalog#14322-H08E; Q9H492-1; Met1-Phe121). LC3A / MAP1LC3A specific IgG was purified by Human LC3A / MAP1LC3A affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-MAP1LC3A Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ATG8EALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-LC3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-LC3AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MAP1ALC3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MAP1BLC3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
MAP1LC3A Background Information
LC3A, also known as MAP1LC3A, is one of the light chain subunits that functions together with both MAP1A and/or MAP1B. MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. As a light chain subunit, MAP1LC3A has an important part in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP1LC3A is expressed as two alternatively spliced isoforms that are expressed in testis, brain, heart, liver and skeletal muscle, but are absent in thymus and peripheral blood leukocytes.
Full Name
microtubule-associated protein 1 light chain 3 alpha
References
He H., et al.,(2003), Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B. J. Biol. Chem. 278:29278-29287. Deloukas P., et al., (2001), The DNA sequence and comparative analysis of human chromosome 20.Nature 414:865-871. Bechtel S., et al.,(2007), The full-ORF clone resource of the German cDNA consortium.BMC Genomics 8:399-399.
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