Anti-ENSA Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-ENSA Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-ENSA magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-ENSA Magnetic Beads-IP Kit Product Description
The Anti-ENSA magnetic Beads, conjugated with Anti-ENSA antibody, are used for immuneprecipitation (IP) of ENSA proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ENSA proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ENSA proteins are dissociated from the magnetic beads using an elution buffer. Anti-ENSA Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human ENSA / Endosulfine alpha protein (Catalog#14321-H07E)
Species Reactivity
Human ENSA / Endosulfine alpha
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human ENSA / Endosulfine alpha (rh ENSA / Endosulfine alpha; Catalog#14321-H07E; O43768-1; Met1-Glu121). ENSA / Endosulfine alpha specific IgG was purified by Human ENSA / Endosulfine alpha affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-ENSA Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ARPP-19eALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
ENSA Background Information
Endosulfine alpha, also known as ENSA, belongs to the endosulfine family. It is a highly conserved cAMP-regulated phosphoprotein (ARPP) family. Endosulfine alpha is widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. As a protein phosphatase inhibitor, ENSA specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase By similarity. Endosulfine alpha also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.
Full Name
endosulfine alpha
References
Ye M. et al., 2001, Genome Res. 10 (10): 1546-60. Apiou F. et al., 1999, Diabetes 48 (9): 1873-6. Lennon G. et al., 1997, Genome Res. 6 (9): 791-806.
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