Anti-COL4A3BP Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-COL4A3BP Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-COL4A3BP magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-COL4A3BP Magnetic Beads-IP Kit Product Description
The Anti-COL4A3BP magnetic Beads, conjugated with Anti-COL4A3BP antibody, are used for immuneprecipitation (IP) of COL4A3BP proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing COL4A3BP proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound COL4A3BP proteins are dissociated from the magnetic beads using an elution buffer. Anti-COL4A3BP Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human COL4A3BP protein (Catalog#14306-H20B)
Species Reactivity
Human COL4A3BP
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human COL4A3BP (rh COL4A3BP; Catalog#14306-H20B; Q9Y5P4-1; Met1-Phe598). COL4A3BP specific IgG was purified by Human COL4A3BP affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-COL4A3BP Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CERTALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CERTLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GPBPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-STARD11ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
COL4A3BP Background Information
COL4A3BP is a member of the StarD2 subfamily. It contains a pleckstrin homology domain at its amino terminus and a START domain towards the end of the molecule. COL4A3BP has a lipid-binding domain that mediates intracellular trafficking of ceramide in a non-vesicular manner. One isoform of COL4A3BP is also involved in ceramide intracellular transport. COL4A3BP specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. An autoimmune response directed at this antigen can cause goodpasture disease.
Full Name
collagen, type IV, alpha 3 (Goodpasture antigen) binding protein
References
Rual JF, et al. (2005) Towards a proteome-scale map of the human protein-protein interaction network. Nature. 437(7062):1173-8. Granero F, et al. (2005) A human-specific TNF-responsive promoter for Goodpasture antigen-binding protein. FEBS J. 272(20):5291-305. Longo I, et al. (2006) Autosomal recessive Alport syndrome: an in-depth clinical and molecular analysis of five families. Nephrol Dial Transplant. 21(3):665-71.
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