Anti-ARD1A Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-ARD1A Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-ARD1A magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-ARD1A Magnetic Beads-IP Kit Product Description
The Anti-ARD1A magnetic Beads, conjugated with Anti-ARD1A antibody, are used for immuneprecipitation (IP) of ARD1A proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ARD1A proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ARD1A proteins are dissociated from the magnetic beads using an elution buffer. Anti-ARD1A Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human ARD1 protein (Catalog#14293-H20B)
Species Reactivity
Human ARD1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human ARD1 (rh ARD1; Catalog#14293-H20B; P41227; Met1-Ser235). ARD1 specific IgG was purified by Human ARD1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-ARD1A Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ARD1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ARD1AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ARD1PALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-DXS707ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MCOPS1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NAA10ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NATDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-OGDNSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TE2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
ARD1A Background Information
ARD1 is a member of the 2-kDa ARF protein family. It is a multifunctional protein. ARD1 has an 18-kDa ADP-ribosylation factor (ARF) domain at the C-terminus (amino acids 43-574), and a 46-kDa N-terminal domain (amino acids 1-42). The C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes. ARD1 and NAT1 genes are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating type locus HML, for sporulation, and for entry into G. Recombinant ARD1 (amino acids 1-574) or its RING finger domain (amino acids 1-11) produced polyubiquitylated proteins when incubated in vitro with a mammalian E1, an E2 enzyme, ATP, and ubiquitin.
Full Name
N(alpha)-acetyltransferase 10, NatA catalytic subunit
References
Tribioli C., et al.,(1994), Isolation of new genes in distal Xq28: transcriptional map and identification of a human homologue of the ARD1 N-acetyl transferase of Saccharomyces cerevisiae. Hum. Mol. Genet. 3:1061-1068. Arnesen T., et al., (2005), Identification and characterization of the human ARD1-NATH protein acetyltransferase complex.Biochem. J. 386:433-443. Ross M.T., et al.,(2005), The DNA sequence of the human X chromosome.Nature 434:325-337.
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