Anti-GSTK1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-GSTK1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-GSTK1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-GSTK1 Magnetic Beads-IP Kit Product Description
The Anti-GSTK1 magnetic Beads, conjugated with Anti-GSTK1 antibody, are used for immuneprecipitation (IP) of GSTK1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing GSTK1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound GSTK1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-GSTK1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human GSTK1 Protein (Catalog#14291-HNAE)
Species Reactivity
Human GSTK1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human GSTK1 (rh GSTK1; Catalog#14291-HNAE; Q9Y2Q3-1; Gly2-Leu226). GSTK1 specific IgG was purified by Human GSTK1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-GSTK1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-GSTALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GST13ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GST13-13ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GSTK1-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-hGSTK1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
GSTK1 Background Information
GSTK1 gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. Glutathione S-transferases (GSTs) are a family of enzymes that catalyze a variety of reactions in both eukaryotes and prokaryotes. They catalyze the conjugation of reduced glutathione with potentially toxic, xenobiotic substrates, thus aiding excretion from the body. GSTK1(glutathione S-transferase kappa 1) is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. GSTK1 functions in cellular detoxification.
Full Name
glutathione S-transferase kappa 1
References
Zhang QH, et al. (2001) Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. Genome Res. 10(10):1546-60. Morel F, et al. (2004) Gene and protein characterization of the human glutathione S-transferase kappa and evidence for a peroxisomal localization. J Biol Chem. 279(16): 16246-53. Jowsey IR, et al. (2003) Biochemical and genetic characterization of a murine class Kappa glutathione S-transferase. Biochem J. 373(Pt 2):559-69.
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