Anti-CPNE1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CPNE1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CPNE1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CPNE1 Magnetic Beads-IP Kit Product Description
The Anti-CPNE1 magnetic Beads, conjugated with Anti-CPNE1 antibody, are used for immuneprecipitation (IP) of CPNE1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CPNE1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CPNE1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CPNE1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human Copine I / CPN1 / CPNE1 Protein (Catalog#14283-HNCB)
Species Reactivity
Human Copine I / CPN1 / CPNE1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human Copine I / CPN1 / CPNE1 (rh Copine I / CPN1 / CPNE1; Catalog#14283-HNCB; Q99829; Met1-Ala537). Copine I / CPN1 / CPNE1 specific IgG was purified by Human Copine I / CPN1 / CPNE1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CPNE1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-COPN1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CPN1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CPNE1 Background Information
Copine I, also known as CPN1, is a member of the copine family. Copine I is a calcium-dependent membrane-binding protein which has a wide tissue distribution. Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. Copine I contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus, while it does not contain a predicted signal sequence or transmembrane domains. Copine I may function in membrane trafficking.
References
Cowland JB, et al. (2003) Tissue expression of copines and isolation of copines I and III from the cytosol of human neutrophils. J Leukoc Biol. 74(3):379-88. Tomsig JL, et al. (2003) Identification of targets for calcium signaling through the copine family of proteins. Characterization of a coiled-coil copine-binding motif. J Biol Chem. 278 (12):10048-54. Strausberg RL, et al. (2003) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.
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