Anti-DARS Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-DARS Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-DARS magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-DARS Magnetic Beads-IP Kit Product Description
The Anti-DARS magnetic Beads, conjugated with Anti-DARS antibody, are used for immuneprecipitation (IP) of DARS proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing DARS proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound DARS proteins are dissociated from the magnetic beads using an elution buffer. Anti-DARS Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human DARS protein (Catalog#14278-H07E)
Species Reactivity
Human DARS
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human DARS (rh DARS; Catalog#14278-H07E; P14868; Met1-Pro501). DARS specific IgG was purified by Human DARS affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-DARS Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-aspRSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HBSLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
DARS Background Information
Aspartate tRNA ligase 1, also known as DARS, is part of a multienzyme complex of aminoacyl-tRNA synthetases. It belongs to the class-II aminoacyl-tRNA synthetase family. DARS charges its cognate tRNA with aspartate during protein biosynthesis. DARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid(AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
Full Name
aspartyl-tRNA synthetase
References
Escalante C, et al. (1993) Expression of human aspartyl-tRNA synthetase in Escherichia coli. Functional analysis of the N-terminal putative amphiphilic helix. J Biol Chem. 268(8): 6014-23. Maruyama K, et al. (1994) Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene. 138(1-2):171-4. Reed VS, et al. (1995) Mechanisms of the transfer of aminoacyl-tRNA from aminoacyl-tRNA synthetase to the elongation factor 1 alpha. J Biol Chem. 269(52):32932-6.
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