Anti-ENO3 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-ENO3 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-ENO3 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-ENO3 Magnetic Beads-IP Kit Product Description
The Anti-ENO3 magnetic Beads, conjugated with Anti-ENO3 antibody, are used for immuneprecipitation (IP) of ENO3 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ENO3 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ENO3 proteins are dissociated from the magnetic beads using an elution buffer. Anti-ENO3 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human ENO3 / beta-enolase Protein (Catalog#14270-H07E)
Species Reactivity
Human ENO3 / beta-enolase
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human ENO3 / beta-enolase (rh ENO3 / beta-enolase; Catalog#14270-H07E; AAH17249.1; Met1-Lys434).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-ENO3 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-GSD13ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MSEALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
ENO3 Background Information
ENO3 is one of the three enolase isoenzymes found in mammals. As a homodimer, ENO3 is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in ENO3 gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for ENO3 gene that differ only in their 5' UTR. ENO3 may play a role in muscle development and regeneration. It appears to have a function in striated muscle development and regeneration.
Full Name
enolase 3 (beta, muscle)
References
Peshavaria M, et al. (1989) Structure of human muscle (beta) enolase mRNA and protein deduced from a genomic clone. Nucleic Acids Res. 17(21):8862. Calì L, et al. (1990) Nucleotide sequence of a cDNA encoding the human muscle-specific enolase (MSE). Nucleic Acids Res. 18(7):1893. Peshavaria M, et al. (1991) Molecular structure of the human muscle-specific enolase gene (ENO3). Biochem J. 275(2):427-33.
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