Anti-CKAP1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CKAP1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CKAP1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CKAP1 Magnetic Beads-IP Kit Product Description
The Anti-CKAP1 magnetic Beads, conjugated with Anti-CKAP1 antibody, are used for immuneprecipitation (IP) of CKAP1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CKAP1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CKAP1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CKAP1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human TBCB protein (Catalog#14260-H07E)
Species Reactivity
Human TBCB
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human TBCB (rh TBCB; Catalog#14260-H07E; Q99426; Met1-Ile244). TBCB specific IgG was purified by Human TBCB affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CKAP1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CG22ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CKAP1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CKAPIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CKAP1 Background Information
Tubulin-folding cofactor B, also known as TBCB, belongs to the TBCB family. It contains 1 CAP-Gly domain and can be detected in most tissues. TBCB binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway. The cytoskeleton is composed of 3 structural elements: actin filaments, microtubules, and intermediate filaments. TBCB is involved in regulation of tubulin heterodimer dissociation. It may function as a negative regulator of axonal growth.
Full Name
tubulin folding cofactor B
References
Feingold EA, et al. (2003) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903. Tian G, et al. (1997) Tubulin subunits exist in an activated conformational state generated and maintained by protein cofactors. J Cell Biol. 138(4):821-32. Wolz W, et al. (1997) A complex satellite DNA polymorphism flanking the human ryanodine receptor gene (RYR1). Cytogenet Cell Genet. 72(2-3):215-6.
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