Anti-UMP / CMP kinase Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-UMP / CMP kinase Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-UMP / CMP kinase magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-UMP / CMP kinase Magnetic Beads-IP Kit Product Description
The Anti-UMP / CMP kinase magnetic Beads, conjugated with Anti-UMP / CMP kinase antibody, are used for immuneprecipitation (IP) of UMP / CMP kinase proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing UMP / CMP kinase proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound UMP / CMP kinase proteins are dissociated from the magnetic beads using an elution buffer. Anti-UMP / CMP kinase Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CMPK1 protein (Catalog#14258-H07B)
Species Reactivity
Human CMPK1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CMPK1 (rh CMPK1; Catalog#14258-H07B; P30085; Met1-Gly196). CMPK1 specific IgG was purified by Human CMPK1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-UMP / CMP kinase Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CKALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CMKALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CMPKALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-UMKALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-UMP-CMPKALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-UMPKALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
UMP / CMP kinase Background Information
CMPK1 plays a key role in the maintenance of pyrimidine nucleotide pool profile and for the metabolism of pyrimidine analogs in cells. It catalyzes the phosphoryl transfer from ATP to UMP, CMP, and deoxy-CMP (dCMP), resulting in the formation of ADP and the corresponding nucleoside diphosphate. CMPK1 also has a significant role in the activation of pyrimidine analogs, which are clinically useful anti-cancer and anti-viral drugs. In the meanwhile, CMPK1 functions in cellular nucleic acid biosynthesis.
Full Name
cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
References
Liou J, et al. (2004) Phosphorylation of Cytidine, Deoxycytidine, and Their Analog Monophosphates by Human UMP/CMP Kinase is Differentially Regulated by ATP and Magnesium. Molecular Pharmacology. 67(3):806-14. Gerhard DS, et al. (2004) The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC). Genome Research. 14(10B):2121-7. Segura-Pena D, et al. (2004) Substrate-induced Conformational Changes in Human UMP/CMP Kinase. Journal of Biological Chemistry. 279(32):33882-9.
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