Anti-GSTZ1 Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-GSTZ1 Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-GSTZ1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-GSTZ1 Magnetic Beads-IP Kit Product Description
The Anti-GSTZ1 magnetic Beads, conjugated with Anti-GSTZ1 antibody, are used for immuneprecipitation (IP) of GSTZ1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing GSTZ1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound GSTZ1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-GSTZ1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human GSTZ1 protein (Catalog#14237-H07E)
Species Reactivity
Human GSTZ1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human GSTZ1 (rh GSTZ1; Catalog#14237-H07E; NP_665877.1; Met1-Ala216). GSTZ1 specific IgG was purified by Human GSTZ1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-GSTZ1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-GSTZ1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GSTZ1-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MAAIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MAIALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
GSTZ1 Background Information
GSTZ1 gene is a member of the glutathione S-transferase (GSTs) super-family which encodes multifunctional enzymes important in the detoxification of electrophilic molecules, including carcinogens, mutagens, and several therapeutic drugs, by conjugation with glutathione. GSTZ1 is a bifunctional protein which has minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole and maleylacetoacetate isomerase activity. GSTZ1 catalyzes the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid. GSTZ1 participates in the catabolism of phenylalanine and tyrosine. Thus defects in GSTZ1 cause harsh metabolic disorders including alkaptonuria, phenylketonuria and tyrosinaemia.
Full Name
glutathione S-transferase zeta 1
References
Tong Z. et al., 1999, Chem Res Toxicol. 11 (11): 1332-8. Tong Z. et al., 1999, Biochem J. 331 (2): 371-4. Ketterer B. 2001, Chem Biol Interact. 138 (1): 27-42.