Anti-Iba1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Iba1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Iba1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Iba1 Magnetic Beads-IP Kit Product Description
The Anti-Iba1 magnetic Beads, conjugated with Anti-Iba1 antibody, are used for immuneprecipitation (IP) of Iba1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Iba1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Iba1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-Iba1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human IBA1 / AIF1 protein (Catalog#14227-H07E)
Species Reactivity
Human IBA1 / AIF1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human IBA1 / AIF1 (rh IBA1 / AIF1; Catalog#14227-H07E; P55008-1; Met1-Pro147). IBA1 / AIF1 specific IgG was purified by Human IBA1 / AIF1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Iba1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-AIF-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-IBA1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-IRT-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-IRT1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Iba1 Background Information
AIF1, also known as IBA1, is an actin-binding protein. AIF1 is expressed selectively in human macrophage-like cell lines, and in a subset of CD68(+) macrophages in the interstitial and perivascular spaces of human heart allografts. It is expressed in macrophages and neutrophils. AIF1 enhances membrane ruffling and RAC activation. AIF1 enhances the actin-bundling activity of LCP1. It also enhances lymphocyte migration. AIF1 may play a role in macrophage activation and function. It binds calcium and plays a role in RAC signaling and in phagocytosis. It promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes.
Full Name
allograft inflammatory factor 1
References
Albertella M.R., et al.,(1994), Characterization of a novel gene in the human major histocompatibility complex that encodes a potential new member of the I kappa B family of proteins. Hum. Mol. Genet. 3:793-799. Holzinger I., et al., (1995), Cloning and genomic characterization of LST1: a new gene in the human TNF region.Immunogenetics 42:315-322. Autieri M.V., et al.,(1996), cDNA cloning of human allograft inflammatory factor-1: tissue distribution, cytokine induction, and mRNA expression in injured rat carotid arteries.Biochem. Biophys. Res. Commun. 228:29-37.
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