Anti-CAPG Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CAPG Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CAPG magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CAPG Magnetic Beads-IP Kit Product Description
The Anti-CAPG magnetic Beads, conjugated with Anti-CAPG antibody, are used for immuneprecipitation (IP) of CAPG proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CAPG proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CAPG proteins are dissociated from the magnetic beads using an elution buffer. Anti-CAPG Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CAPG / AFCP protein (Catalog#14213-HNAE)
Species Reactivity
Human CAPG / AFCP
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CAPG / AFCP (rh CAPG / AFCP; Catalog#14213-HNAE; AAH14549.1; Met1-Lys348). CAPG / AFCP specific IgG was purified by Human CAPG / AFCP affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CAPG Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-AFCPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HEL-S-66ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MCPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CAPG Background Information
CAPG, also known as actin Regulatory Protein CAPG, is a a member of the gelsolin/villin family. Members of this family are actin-regulatory proteins. CAPG reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, CAPG contributes to the control of actin-based motility in non-muscle cells. CAPG may also play an important role in macrophage function.
Full Name
capping protein (actin filament), gelsolin-like
References
Watari A. et al., 2007, Oncogene. 25 (56): 7373-80. De Corte V. et al., 2005, J Cell Sci. 117 (22): 5283-92. Van Impe K. et al., 2003, J Biol Chem. 278 (20): 17945-52.
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