Anti-Aspartate Aminotransferase Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-Aspartate Aminotransferase Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Aspartate Aminotransferase magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Aspartate Aminotransferase Magnetic Beads-IP Kit Product Description
The Anti-Aspartate Aminotransferase magnetic Beads, conjugated with Anti-Aspartate Aminotransferase antibody, are used for immuneprecipitation (IP) of Aspartate Aminotransferase proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Aspartate Aminotransferase proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Aspartate Aminotransferase proteins are dissociated from the magnetic beads using an elution buffer. Anti-Aspartate Aminotransferase Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-Aspartate Aminotransferase Antibody(
14196-T52)
Immunogen
Recombinant Human Aspartate aminotransferase / GOT1 protein (Catalog#14196-H07E)
Species Reactivity
Human Aspartate aminotransfera
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human Aspartate aminotransferase / GOT1 (rh Aspartate aminotransferase / GOT1; Catalog#14196-H07E; P17174; Met1-Gln413). Aspartate aminotransferase / GOT1 specific IgG was purified by Human Aspartate aminotransferase / GOT1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Aspartate Aminotransferase Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ASTQTL1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-cAspATALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-cCATALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GIG18ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Aspartate Aminotransferase Background Information
Aspartate aminotransferase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, aspartate aminotransferase and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. There is a rare in-frame deletion in aspartate aminotransferase gene, which inactivates cytosolic aspartate aminotransferase(cAST) enzyme in the Old Order Amish. This may help to understand structure and function of the enzyme and would be useful for predicting serum aspartate AST levels.
Full Name
glutamic-oxaloacetic transaminase 1, soluble
References
Shen H, et al. (2011) Genome-wide association study identifies genetic variants in GOT1 determining serum aspartate aminotransferase levels. J Hum Genet. 56(11):801-5. Doonan S, et al. (1985) Structural and genetic relationships between cytosolic and mitochondrial isoenzymes. Int J Biochem. 16(12):1193-9. Panteghini M. (1990) Aspartate aminotransferase isoenzymes. Clin Biochem. 23(4):311-9. Bousquet-Lemercier B, et al. (1990) Properties of human liver cytosolic aspartate aminotransferase mRNAs generated by alternative polyadenylation site selection. Biochemistry. 29(22):5293-9.