Anti-PPIL1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-PPIL1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-PPIL1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-PPIL1 Magnetic Beads-IP Kit Product Description
The Anti-PPIL1 magnetic Beads, conjugated with Anti-PPIL1 antibody, are used for immuneprecipitation (IP) of PPIL1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PPIL1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PPIL1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-PPIL1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human PPIL1 protein (Catalog#14163-H08E)
Species Reactivity
Human PPIL1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human PPIL1 (rh PPIL1; Catalog#14163-H08E; Q9Y3C6; Met1-Gly166). PPIL1 specific IgG was purified by Human PPIL1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-PPIL1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CGI-124ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CYPL1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-hCyPXALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PPIaseALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
PPIL1 Background Information
PPIL1 is a member of the cyclophilin family. Cyclophilins are well conserved and ubiquitous. Members of cyclophilin family take an significant part in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. PPIL1 is a peptidylprolyl isomerase(PPIase). It increases the folding of proteins and catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIL1 is involved in proliferation of cancer cells through modulation of phosphorylation of stathmin. It is a novel molecular target for colon-cancer therapy.
Full Name
peptidylprolyl isomerase (cyclophilin)-like 1
References
Xu C. et al., 2005, J Biomol NMR. 31 (2): 179-80. Ozaki K. et al., 1997, Cytogenet Cell Genet. 72 (2-3): 242-5. Mann SS. et al., 1999, Cytogenet Cell Genet. 83 (3-4): 228-9.
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