Anti-NDRG1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-NDRG1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-NDRG1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-NDRG1 Magnetic Beads-IP Kit Product Description
The Anti-NDRG1 magnetic Beads, conjugated with Anti-NDRG1 antibody, are used for immuneprecipitation (IP) of NDRG1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing NDRG1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound NDRG1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-NDRG1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human NDRG1 / CAP43 protein (Catalog#14119-H08E)
Species Reactivity
Human NDRG1 / CAP43
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human NDRG1 / CAP43 (rh NDRG1 / CAP43; Catalog#14119-H08E; Q92597; Met1-Cys394). NDRG1 / CAP43 specific IgG was purified by Human NDRG1 / CAP43 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-NDRG1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CAP43ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CMT4DALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-DRG-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-DRG1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-GC4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HMSNLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NDR1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NMSLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PROXY1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-RIT42ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-RTPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TARG1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TDD5ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
NDRG1 Background Information
NDRG1 gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. NDRG1 is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. NDRG1 is necessary for p53-mediated caspase activation and apoptosis. Mutations in NDRG1 gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. NDRG1 is a stress-responsive protein involved in hormone responses, cell growth, and differentiation. It acts as a tumor suppressor in many cell types.
Full Name
N-myc downstream regulated 1
References
Kachhap SK, et al. (2007) The N-Myc down regulated Gene1 (NDRG1) Is a Rab4a effector involved in vesicular recycling of E-cadherin. PLoS ONE. 2(9):e844. Zhang J, et al. (2008) Human differentiation-related gene NDRG1 is a Myc downstream-regulated gene that is repressed by Myc on the core promoter region. Gene. 417(1-2):5-12. Kokame K, et al. (1997) Homocysteine-respondent genes in vascular endothelial cells identified by differential display analysis. GRP78/BiP and novel genes. J Biol Chem. 271(47): 29659-65.
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