Anti-RUVBL1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-RUVBL1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-RUVBL1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-RUVBL1 Magnetic Beads-IP Kit Product Description
The Anti-RUVBL1 magnetic Beads, conjugated with Anti-RUVBL1 antibody, are used for immuneprecipitation (IP) of RUVBL1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing RUVBL1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound RUVBL1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-RUVBL1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human RUVBL1 / RVB1 Protein (Catalog#14074-H07B)
Species Reactivity
Human RUVBL1 / RVB1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human RUVBL1 / RVB1 (rh RUVBL1 / RVB1; Catalog#14074-H07B; Q9Y265-1; Met1-Lys456). RUVBL1 / RVB1 specific IgG was purified by Human RUVBL1 / RVB1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-RUVBL1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ECP54ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-INO80HALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NMP238ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PONTINALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-Pontin52ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-RVB1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TIH1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TIP49ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TIP49AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
RUVBL1 Background Information
RUVBL1, also known as RVB1, is a component of the NuA4 histone acetyltransferase complex and belongs to the RuvB family. RUVBL1 is ubiquitously expressed with high expression in heart, skeletal muscle and testis. It possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. RUVBL1 is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. It also is essential for cell proliferation. RUVBL1 may be able to bind plasminogen at cell surface and enhance plasminogen activation.
Full Name
RuvB-like AAA ATPase 1
References
Bauer A, et al. (1998) Pontin52, an interaction partner of beta-catenin, binds to the TATA box binding protein. Proc Natl Acad Sci. 95(25):14787-92. Ewing, et al. (2007) Large-scale mapping of human protein-protein interactions by mass spectrometry. Mol Syst Biol. 3(1):89. Puri T, et al. (2007) Dodecameric structure and ATPase activity of the human TIP48/TIP49 complex. J Mol Biol. 366(1):179-92.
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