Anti-RPRD1B Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-RPRD1B Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-RPRD1B magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-RPRD1B Magnetic Beads-IP Kit Product Description
The Anti-RPRD1B magnetic Beads, conjugated with Anti-RPRD1B antibody, are used for immuneprecipitation (IP) of RPRD1B proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing RPRD1B proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound RPRD1B proteins are dissociated from the magnetic beads using an elution buffer. Anti-RPRD1B Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human RPRD1B protein (Catalog#14027-H07H)
Species Reactivity
Human RPRD1B
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human RPRD1B (rh RPRD1B; Catalog#14027-H07H; AAH33629.1; Met1-Asp326). RPRD1B specific IgG was purified by Human RPRD1B affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-RPRD1B Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-C20orf77ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CREPTALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-dJ1057B20.2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-NET60ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
RPRD1B Background Information
RPRD1B, together with RPRD1A, can accompany RNAP II from promoter regions to 3'-untranslated regions during transcription in vivo, predominantly interact with phosphorylated RNAP II, and can reduce CTD S5- and S7-phosphorylated RNAP II at target gene promoters. RNA polymerase II C-terminal domain (CTD) phosphorylation is important for various transcription-related processes. RPRD1B is a transcriptional regulator which enhances expression of CCND1. It also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A.
Full Name
regulation of nuclear pre-mRNA domain containing 1B
References
Ni Z. et al., 2011, Transcription. 2 (5): 237-42. Kristensen AR. et al., 2012, Nat Methods. 9 (9): 907-9. Woods NT. et al., 2012, Sci Signal.5 (242): rs6.
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