Anti-EBAG9 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-EBAG9 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-EBAG9 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-EBAG9 Magnetic Beads-IP Kit Product Description
The Anti-EBAG9 magnetic Beads, conjugated with Anti-EBAG9 antibody, are used for immuneprecipitation (IP) of EBAG9 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing EBAG9 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound EBAG9 proteins are dissociated from the magnetic beads using an elution buffer. Anti-EBAG9 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human RCAS1 / EBAG9 Protein (Catalog#14012-H07E)
Species Reactivity
Human RCAS1 / EBAG9
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human RCAS1 / EBAG9 (rh RCAS1 / EBAG9; Catalog#14012-H07E; O00559; Arg28-Ser213). RCAS1 / EBAG9 specific IgG was purified by Human RCAS1 / EBAG9 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-EBAG9 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-EB9ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PDAFALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
EBAG9 Background Information
RCAS1, also known as EBAG9, is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. RCAS1 gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of RCAS1 gene. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for RCAS1 gene. EBAG9 may participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.
Full Name
estrogen receptor binding site associated, antigen, 9
References
Ohshima K. et al., 2001, Clin Exp Immunol. 123 (3): 481-6. Ikeda K. et al., 2000, Biochem Biophys Res Commun. 273 (2): 654-60. Nakashima M. et al., 1999, Nat Med. 5 (8): 938-42.
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