Anti-MID1IP1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-MID1IP1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-MID1IP1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-MID1IP1 Magnetic Beads-IP Kit Product Description
The Anti-MID1IP1 magnetic Beads, conjugated with Anti-MID1IP1 antibody, are used for immuneprecipitation (IP) of MID1IP1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing MID1IP1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound MID1IP1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-MID1IP1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human MID1IP1 protein (Catalog#14005-H07E)
Species Reactivity
Human MID1IP1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human MID1IP1 (rh MID1IP1; Catalog#14005-H07E; Q9NPA3; Met1-His183). MID1IP1 specific IgG was purified by Human MID1IP1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-MID1IP1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-G12-likeALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MIG12ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-S14RALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-STRAIT11499ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-THRSPLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
MID1IP1 Background Information
MID1IP1 belongs to the SPOT14 family. It is a homodimer in the absence of THRSP. MID1IP1 interacts with ACACA and ACACB. It plays a role in the regulation of lipogenesis in liver. It up-regulates ACACA enzyme activity. MID1IP1 is required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol and phospholipid. MID1IP1 is involved in stabilization of microtubules. Its interaction with THRSP interferes with ACACA binding.
Full Name
MID1 interacting protein 1
References
Aipoalani DL. et al., 2010, Endocrinology. 151 (5): 2071-7. Colbert CL. et al., 2010, Proc Natl Acad Sci. 107 (44): 18820-5. Inoue J. et al., 2011, Mol Endocrinol. 25 (6): 995-1005.
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