Anti-CKMT1A Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CKMT1A Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CKMT1A magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CKMT1A Magnetic Beads-IP Kit Product Description
The Anti-CKMT1A magnetic Beads, conjugated with Anti-CKMT1A antibody, are used for immuneprecipitation (IP) of CKMT1A proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CKMT1A proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CKMT1A proteins are dissociated from the magnetic beads using an elution buffer. Anti-CKMT1A Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CKMT1A protein (Catalog#13924-H07B1)
Species Reactivity
Human CKMT1A
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CKMT1A (rh CKMT1A; Catalog#13924-H07B1; P12532-1; Ala40-His417). CKMT1A specific IgG was purified by Human CKMT1A affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CKMT1A Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CKMTALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CKMT1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CKMT1AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CKMT1A Background Information
CKMT1A belongs to the ATP:guanido phosphotransferase family. It contains 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. CKMT1A gene is one of two genes which encode the ubiquitous mitochondrial creatine kinase (CKMT1). CKMT1 is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK (CKMT2) and ubiquitous MtCK, encoded by separate genes. CKMT1 occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Ubiquitous mitochondrial creatine kinase has 8% homology with the coding exons of sarcomeric CKMT1.
Full Name
creatine kinase, mitochondrial 1A
References
Haas RC, et al. (1989) Isolation and characterization of the gene and cDNA encoding human mitochondrial creatine kinase. J Biol Chem. 264(5):2890-7. Stachowiak O, et al. (1998) Oligomeric state and membrane binding behaviour of creatine kinase isoenzymes: implications for cellular function and mitochondrial structure. Mol Cell Biochem. 184(1-2):141-51. Lipskaya TY. (2001) Mitochondrial creatine kinase: properties and function. Biochemistry Mosc. 66(10):1098-111.
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