Anti-SAE1 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-SAE1 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-SAE1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-SAE1 Magnetic Beads-IP Kit Product Description
The Anti-SAE1 magnetic Beads, conjugated with Anti-SAE1 antibody, are used for immuneprecipitation (IP) of SAE1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing SAE1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound SAE1 proteins are dissociated from the magnetic beads using an elution buffer. Anti-SAE1 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human SAE1 protein (Catalog#13921-H20B)
Species Reactivity
Human SAE1
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human SAE1 (rh SAE1; Catalog#13921-H20B; Q9UBE0; Met1-Lys346). SAE1 specific IgG was purified by Human SAE1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-SAE1 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-AOS1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HSPC140ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SUA1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-UBLE1AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
SAE1 Background Information
SAE1 belongs to the ubiquitin-activating E1 family. It is a heterodimer that acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It functions as a UBLI E1 ligase mediating the ATP-dependent activation of UBL1. SAE1 binds with UBLE1A and UBLE1B to form a heterodimer which can bind UBL1. SAE1 also regulates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins.
Full Name
SUMO1 activating enzyme subunit 1
References
Gong L, et al. (1999) Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex. FEBS Lett. 448(1):185-9. Lois LM, et al. (2005) Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1. EMBO J. 24(3):439-51. Tatham MH, et al. (2001) Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9. J Biol Chem. 276(38):35368-74.
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