Anti-PARVA Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-PARVA Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-PARVA magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-PARVA Magnetic Beads-IP Kit Product Description
The Anti-PARVA magnetic Beads, conjugated with Anti-PARVA antibody, are used for immuneprecipitation (IP) of PARVA proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PARVA proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PARVA proteins are dissociated from the magnetic beads using an elution buffer. Anti-PARVA Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human Actopaxin protein (Catalog#13919-H09E)
Species Reactivity
Human Actopaxin
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human Actopaxin (rh Actopaxin; Catalog#13919-H09E; Q9NVD7-1; Met1-Glu372). Actopaxin specific IgG was purified by Human Actopaxin affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-PARVA Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CH-ILKBPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MXRA2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
PARVA Background Information
Actopaxin, also known as alpha-parvin, belongs to the parvin family. It is widely expressed, with highest levels in heart, skeletal muscle, kidney and liver. Actopaxin contains 2 CH (calponin-homology) domains and probably plays a role in the regulation of cell adhesion and cytoskeleton organization. It interacts with integrin-linked protein kinase and probably with actin and the LD1 and LD4 motifs of PXN. Actopaxin binds directly to both F-actin and paxillin LD1 and LD4 motifs. Actopaxin also exhibits robust focal adhesion localization in several cultured cell types but is not found along the length of the associated actin-rich stress fibers. It is absent from actin-rich cell-cell adherens junctions.
References
Korenbaum E, et al. (2002) Genomic organization and expression profile of the parvin family of focal adhesion proteins in mice and humans. Gene. 279(1):69-79. Nikolopoulos SN, et al. (2002) Molecular dissection of actopaxin-integrin-linked kinase-Paxillin interactions and their role in subcellular localization. J Biol Chem. 277(2): 1568-75. Tu Y, et al. (2001) A new focal adhesion protein that interacts with integrin-linked kinase and regulates cell adhesion and spreading. J Cell Biol. 153(3): 585-98.
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