Anti-ABHD14B Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-ABHD14B Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-ABHD14B magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-ABHD14B Magnetic Beads-IP Kit Product Description
The Anti-ABHD14B magnetic Beads, conjugated with Anti-ABHD14B antibody, are used for immuneprecipitation (IP) of ABHD14B proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ABHD14B proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ABHD14B proteins are dissociated from the magnetic beads using an elution buffer. Anti-ABHD14B Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human ABHD14B protein (Catalog#13913-H07E)
Species Reactivity
Human ABHD14B
Source
Monoclonal Human Rabbit IgG
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human ABHD14B (rh ABHD14B; Catalog#13913-H07E; Q96IU4-1; Met1-Gln210).
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-ABHD14B Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CIBALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HEL-S-299ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
ABHD14B Background Information
ABHD14B belongs to the AB hydrolase superfamily, ABHD14 family. It can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro) and may interact with TAF1. It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. ABHD14B contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing gene subfamily is comprised of 15 mostly uncharacterized members.
Full Name
abhydrolase domain containing 14B
References
Mehrle A, et al. (2006) The LIFEdb database in 2006. Nucleic Acids Res. 34:D415-8. Wan D, et al. (2004) Large-scale cDNA transfection screening for genes related to cancer development and progression. Proc Natl Acad Sci. 101(44):15724-9. Wiemann S, et al. (2004) From ORFeome to biology: a functional genomics pipeline. Genome Res. 14 (10B):2136-44.
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