Anti-CNPY2 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-CNPY2 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-CNPY2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-CNPY2 Magnetic Beads-IP Kit Product Description
The Anti-CNPY2 magnetic Beads, conjugated with Anti-CNPY2 antibody, are used for immuneprecipitation (IP) of CNPY2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CNPY2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CNPY2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-CNPY2 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human CNPY2 protein (Catalog#13771-H08H)
Species Reactivity
Human CNPY2
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human CNPY2 (rh CNPY2; Catalog#13771-H08H; Q9Y2B0-1; Met1-Ser178). CNPY2 specific IgG was purified by Human CNPY2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-CNPY2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-HP10390ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MSAPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-TMEM4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ZSIG9ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
CNPY2 Background Information
CNPY2 is a novel MIR-interacting protein that enhances neurite outgrowth and increases myosin regulatory light chain. CNPY2 enhances migration of C6 glioma cells through phosphorylation of the myosin regulatory light chain. It is expressed in different tissues, including brain. Overexpression of CNPY2 enhanced the motility of glioma cells measured in matrigel invasion chambers and using a scratch assay. Downregulation of CNPY2 by RNA interference significantly decreased glioma cell migration and phosphorylation of MRLC. Inhibition of the corresponding MRLC kinase by ML-7 did not affect migration of CNPY2-overexpressing cells.
Full Name
canopy FGF signaling regulator 2
References
Trynka G, et al. (2009) Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling. Gut. 58(8):1078-83. Danielsen JM, et al. (2011) Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level. Mol Cell Proteomics. 10(3):M110.003590. Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 44(2):325-40.
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