Anti-Desmoglein-2 Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-Desmoglein-2 Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Desmoglein-2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Desmoglein-2 Magnetic Beads-IP Kit Product Description
The Anti-Desmoglein-2 magnetic Beads, conjugated with Anti-Desmoglein-2 antibody, are used for immuneprecipitation (IP) of Desmoglein-2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Desmoglein-2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Desmoglein-2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-Desmoglein-2 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human DSG2 Protein
Species Reactivity
Human DSG2
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human DSG2 (rh DSG2; ). DSG2 specific IgG was purified by Human DSG2 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Desmoglein-2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-ARVC10ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ARVD10ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CDHF5ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CMD1BBALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HDGCALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Desmoglein-2 Background Information
Desmoglein-2 (Dsg2) is a specific cadherin of the cell-cell contact in cardiac desmosomes. Mutations in the DSG2-gene are regarded to cause arrhythmogenic (right ventricular) cardiomyopathy (ARVC) which is a rare but severe heart muscle disease. The depletion of DSG2 markedly decreased hPSC proliferation and pluripotency marker expression. In addition, DSG2-negative population in hPSCs exhibited a notable suppression in embryonic body and teratoma formation. The actions of DSG2 in regulating the self-renewal and pluripotency of hPSCs were predominantly exerted through the regulation of beta-catenin/Slug-mediated epithelial-to-mesenchymal transition. Which demonstrate that DSG2 is a valuable PSC surface marker that is essential for the maintenance of PSC self-renewal.