Anti-BPHL Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-BPHL Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-BPHL magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-BPHL Magnetic Beads-IP Kit Product Description
The Anti-BPHL magnetic Beads, conjugated with Anti-BPHL antibody, are used for immuneprecipitation (IP) of BPHL proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing BPHL proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound BPHL proteins are dissociated from the magnetic beads using an elution buffer. Anti-BPHL Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human BPHL protein (Catalog#13675-H07E)
Species Reactivity
Human BPHL
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human BPHL (rh BPHL; Catalog#13675-H07E; Q86WA6-1; Ser38-Gln291). BPHL specific IgG was purified by Human BPHL affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-BPHL Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-BPH-RPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-MCNAAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-VACVASEALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
BPHL Background Information
BPHL is a member of the serine protease family. BPHL is expressed large quantities in liver and kidney and in minor quantities in heart, intestine and skeletal muscle. BPHL is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. It catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. BPHL also activates valacyclovir to acyclovir. It may play a role in detoxification processes.
Full Name
biphenyl hydrolase-like (serine hydrolase)
References
Lai L. et al., 2008, J Biol Chem. 283 (14): 9318-27. Davila S. et al., 2010, Genes Immun. 11 (3): 232-8. Hendrickson SL. et al., 2010, PLoS One. 5 (9): e12862.
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