Anti-LAMP2 Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-LAMP2 Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-LAMP2 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-LAMP2 Magnetic Beads-IP Kit Product Description
The Anti-LAMP2 magnetic Beads, conjugated with Anti-LAMP2 antibody, are used for immuneprecipitation (IP) of LAMP2 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing LAMP2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound LAMP2 proteins are dissociated from the magnetic beads using an elution buffer. Anti-LAMP2 Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human LAMP2 / CD107b Protein (Catalog#13555-H08H)
Species Reactivity
Human LAMP2 / CD107b
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human LAMP2 / CD107b (rh LAMP2 / CD107b; Catalog#13555-H08H; NP_054701.1; Met1-Ile375). LAMP2 / CD107b specific IgG was purified by Human LAMP2 / CD107b affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-LAMP2 Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-CD107bALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-LAMP-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-LAMPBALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-LGP110ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
LAMP2 Background Information
LAMP2 (Lysosomal-associated membrane protein 2), also known as CD17b (Cluster of Differentiation 17b), is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. In human, LAMP2, the causative gene of Danon disease, located on chromosome Xq24, encodes the lysosome-associated membrane protein-2 (LAMP-2). LAMP-2 deficiency, or Danon disease, is a rare X-linked lysosomal disease characterized by cardiomyopathy, vacuolar myopathy, and mental retardation. LAMP2 cardiomyopathy is an X-linked and highly progressive myocardial storage disorder associated with diminished survival, which clinically resembles sarcomeric hypertrophic cardiomyopathy.
Full Name
lysosomal-associated membrane protein 2
References
Maron BJ, et al. (2010) Profound left ventricular remodeling associated with LAMP2 cardiomyopathy. Am J Cardiol. 106(8): 1194-6. Di Blasi C, et al. (2008) Danon disease: a novel LAMP2 mutation affecting the pre-mRNA splicing and causing aberrant transcripts and partial protein expression. Neuromuscul Disord. 18(12): 962-6. Echaniz-Laguna A, et al. (2006) Novel Lamp-2 gene mutation and successful treatment with heart transplantation in a large family with Danon disease. Muscle Nerve. 33(3): 393-7.
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