Anti-Calreticulin Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Calreticulin Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Calreticulin magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Calreticulin Magnetic Beads-IP Kit Product Description
The Anti-Calreticulin magnetic Beads, conjugated with Anti-Calreticulin antibody, are used for immuneprecipitation (IP) of Calreticulin proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Calreticulin proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Calreticulin proteins are dissociated from the magnetic beads using an elution buffer. Anti-Calreticulin Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human Calreticulin protein (Catalog#13539-H08H)
Species Reactivity
Human Calreticulin
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human Calreticulin (rh Calreticulin; Catalog#13539-H08H; P27797; Met1-Ala413). Calreticulin specific IgG was purified by Human Calreticulin affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Calreticulin Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-cC1qRALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-CRTALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-HEL-S-99nALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-ROALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SSAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Calreticulin Background Information
Calreticulin is a multifunctional protein. It acts as a main Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. Calreticulin binds Ca2+ ions (a second messenger in signal transduction), rendering it inactive. The Ca2+ is bound with low affinity, but high capacity, and can be released on a signal. Located in storage compartments associated with the endoplasmic reticulum, calreticulin also binds to misfolded proteins and prevents them from being exported from the endoplasmic reticulum to the golgi apparatus. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin reduces the binding of androgen receptor to its hormone-responsive DNA element and inhibits androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Therefore, calreticulin acts as a significant modulator of the regulation of gene transcription by nuclear hormone receptors.
References
Michalak M, et al. (2002) Calreticulin in cardiac development and pathology. Biochim Biophys Acta. 1600(1-2):32-7. Chao MP, et al. (2010) Calreticulin is the dominant pro-phagocytic signal on multiple human cancers and is counterbalanced by CD47. Sci Transl Med. 2(63):63ra94. Andrin, C, et al. (1998) Interaction between a Ca2+-binding protein calreticulin and perforin, a component of the cytotoxic T-cell granules. Biochemistry. 37(29):10386-94.
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