Anti-Phosphoserine phosphatase Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Phosphoserine phosphatase Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Phosphoserine phosphatase magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Phosphoserine phosphatase Magnetic Beads-IP Kit Product Description
The Anti-Phosphoserine phosphatase magnetic Beads, conjugated with Anti-Phosphoserine phosphatase antibody, are used for immuneprecipitation (IP) of Phosphoserine phosphatase proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Phosphoserine phosphatase proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Phosphoserine phosphatase proteins are dissociated from the magnetic beads using an elution buffer. Anti-Phosphoserine phosphatase Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-Phosphoserine phosphatase Antibody(
13503-T52)
Immunogen
Recombinant Human PSPH protein (Catalog#13503-HNAE)
Species Reactivity
Human PSPH
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human PSPH (rh PSPH; Catalog#13503-HNAE; P78330; Met1-Glu225). PSPH specific IgG was purified by Human PSPH affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Phosphoserine phosphatase Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-PSPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-PSPHDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Phosphoserine phosphatase Background Information
Phosphoserine phosphatase (PSPH) belongs to a subfamily of the phosphotransferases. PSPH is the rate-limiting enzyme in l-serine biosynthesis. It has previously been found that Phosphoserine phosphatase (PSPH) plays a role in epidermal homeostasis. Phosphoserine phosphatase (PSP) catalyzes the hydrolysis of phosphoserine to serine. Phosphoserine phosphatase (PSPH) expression has been examined in human-mouse somatic cell hybrids retaining different combination of human chromosomes. Phosphoserine phosphatase (PSPH) is expressed throughout the proliferative layer of the epidermis and hair follicles in rodent and human skin and is highly induced in SCC. In keratinocytes, Phosphoserine phosphatase (PSPH) is a cytoplasmic protein that primarily localizes to endosomes and is present primarily as a homodimer. Knock down of Phosphoserine phosphatase (PSPH) dramatically diminished SCC cell proliferation and cyclin D1 levels in the presence of exogenous of l-serine production suggesting a non-canonical role for Phosphoserine phosphatase (PSPH) in epithelial carcinogenesis. Phosphoserine phosphatase (PSPH) is highly induced in proliferative normal keratinocytes and in skin tumors. Phosphoserine phosphatase (PSPH) appears to be critical for the proliferation of SCC cells; however, this phenomenon may not involve the phosphoserine metabolic pathway.
Full Name
phosphoserine phosphatase
References
Bachelor MA, et al. (2011) L-3-Phosphoserine phosphatase (PSPH) regulates cutaneous squamous cell carcinoma proliferation independent of L-serine biosynthesis. J Dermatol Sci. 63(3): 164-72. Koch GA, et al. (1983) Assignment of the human phosphoserine phosphatase gene (PSP) to the pter leads to q22 region of chromosome 7. Cytogenet Cell Genet. 35(1): 67-9. Jaeken J, et al. (1997) Phosphoserine phosphatase deficiency in a patient with Williams syndrome. J Med Genet. 34(7): 594-6.
新浪微博
腾讯微博
QQ空间
客服
