Anti-Doublecortin Magnetic Beads-IP Kit Product Components
| Components | Storage |
| Anti-Doublecortin Magnetic Beads1,3 | 2-8℃ for 12 months |
| NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
| 5×TBST（pH7.4） | |
| 1×TBST（pH7.4） | |
| ddH2O | |
| CD166 Positive Cell Lysate | -20℃ for 12 months |
| Alkaline Elution Buffer | 2-8℃ for 12 months |
| Acidity Elution Buffer | 2-8℃ for 12 months |
| Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Doublecortin magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Doublecortin Magnetic Beads-IP Kit Product Description
The Anti-Doublecortin magnetic Beads, conjugated with Anti-Doublecortin antibody, are used for immuneprecipitation (IP) of Doublecortin proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Doublecortin proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Doublecortin proteins are dissociated from the magnetic beads using an elution buffer. Anti-Doublecortin Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human DCX protein (Catalog#13498-H09E)
Species Reactivity
Human DCX
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human DCX (rh DCX; Catalog#13498-H09E; O43602-2; Ala45-Val150). DCX specific IgG was purified by Human DCX affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Anti-Doublecortin Magnetic Beads Immunoprecipitation (IP) Kit Alternative Names
Anti-DBCNALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-DCALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-LISXALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-SCLHALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit;Anti-XLISALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit
Doublecortin Background Information
DCX (doublecortin, N-GST chimera)contains 2 doublecortin domains and belongs to the doublecortin family. It is highly expressed in neuronal cells of fetal brain, but not expressed in other fetal tissues. In the adult, it is highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. DCX is a microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. It may act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. DCX may in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. It may be part with LIS-1 of a overlapping, but distinct, signaling pathways that promote neuronal migration. Defects in DCX are the cause of lissencephaly X-linked type 1 and subcortical band heterotopia X-linked.
References
Des Portes V, et al. (1998) A novel CNS gene required for neuronal migration and involved in X-linked subcortical laminar heterotopia and lissencephaly syndrome. Cell. 92:51-61. Gleeson J G, et al. (998) Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein. Cell. 92:63-72. Ross M T, et al. (2005) The DNA sequence of the human X chromosome. Nature. 434:325-37.
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