Anti-Calumenin Magnetic Beads-IP Kit Product Components
Components | Storage |
Anti-Calumenin Magnetic Beads1,3 | 2-8℃ for 12 months |
NP40 Cell Lysis Buffer2 | -20℃ for 12 months |
5×TBST(pH7.4) | |
1×TBST(pH7.4) | |
ddH2O | |
CD166 Positive Cell Lysate | -20℃ for 12 months |
Alkaline Elution Buffer | 2-8℃ for 12 months |
Acidity Elution Buffer | 2-8℃ for 12 months |
Neutralization Buffer | 2-8℃ for 12 months |
[1] The IP KIT contains anti-Calumenin magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.
[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.
Anti-Calumenin Magnetic Beads-IP Kit Product Description
The Anti-Calumenin magnetic Beads, conjugated with Anti-Calumenin antibody, are used for immuneprecipitation (IP) of Calumenin proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Calumenin proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Calumenin proteins are dissociated from the magnetic beads using an elution buffer. Anti-Calumenin Magnetic Beads-IP Kit Antibody Information
Immunogen
Recombinant Human Calumenin / CALU Protein (Catalog#13486-H08H)
Species Reactivity
Human Calumenin / CALU
Source
Polyclonal Human Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Human Calumenin / CALU (rh Calumenin / CALU; Catalog#13486-H08H; O43852-1; Met1-Arg311). Calumenin / CALU specific IgG was purified by Human Calumenin / CALU affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
Calumenin Background Information
Calumenin belongs to the CREC family. It contains 6 EF-hand domains. Calumenin is expressed in skeletal muscle (at protein level). Calumenin interacts with GGCX and RYR1 in the presence of calcium ions, but not in the presence of EDTA. Calumenin is Involved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. It seems to inhibit gamma-carboxylase GGCX. Calumenin also binds 7 calcium ions with a low affinity and may modulate calcium release from the sarcoplasmic reticulum.
References
Hartley JL, et al. (2001) DNA cloning using in vitro site-specific recombination. Genome Res. 10 (11):1788-95. Vorum H, et al. (2000) Calumenin interacts with serum amyloid P component. FEBS Lett. 465 (2-3):129-34. Vorum H, et al. (1999) n calumenin localizes to the secretory pathway and is secreted to the medium. Exp Cell Res. 248(2):473-81.